Erythropoietin prevents ischemia-reperfusion induced oxidative damage in fetal rat brain

Amaç

The aim of this study was to show the effect of erythropoietin on ischemia-reperfusion induced oxidative damage in fetal rat brain.

Yöntem

Fetal brain ischemia was induced by damping the utero-ovarian artery bilaterally for 20 minutes and reperfusion was achieved by removing the clamps for 30 minutes. in control group, non-injured 19 day pregnant rats were used. in ischemia-reperfusion group, no treatment was given. 0.4 ml of human serum albumin solution and 5000 U/kg recombinant human erithropoietin (r-Hu-EPO) were administered in vehicle and treatment groups 30 min. before ischemia-reperfusion injury. Lipid peroxidation in the brain tissue was determined as thiobarbituric acid reactive substances (TBARS) concentration for each fetal rat. The one-way analysis of variance and post-hoc test were used for statistical analysis.

Bulgular

TBARS increased statistically significant levels in fetal rat brain after ischemia-reperfusion injury comparing to control group. Recombinant human erythropoietin prevented increase in TBARS in ischemia-reperfusion injury.

Sonuç

Recombinant human erythropoietin has been shown to have neuroprotective effect in intrauterine ischemia-reperfusion induced fetal brain damage in rats.

Anahtar Kelimeler

-